Coupled Multispectroscopy Singular Laboratory (Raman-LASPEA)

Infrastructures
Research areas
  • Physical Sciences
Website
http://www.ehu.eus/en/web/sgiker/multiespektroskopia-akoplatuen-laborategi-berezia-aurkezpena
Contact

Dr. Alfredo Sarmiento Phone: 94 601 3533 e-mail: alfredo.sarmiento@ehu.es

Available for external users?
Yes
Access protocol

Conditions for using the service Internal users of the University of the Basque Country / Euskal Herriko Unibertsitatea, in addition to institutions that maintain agreements with the UPV/EHU, are able to take advantage of special prices for the services contracted at the Advanced Research Facilities (SGIker). These special prices are subject to the following clauses: The results obtained cannot be used to obtain any additional gain. If the results obtained from the analyses are published in magazines, congresses or talks, etc., express reference muat be provided to the technician and the SGIker service where the results were obtained in the acknowledgements section. Parties to the contract are duty bound to provide the technician with a copy of the relevant article, abstract or book chapter. General rules Access to the unit of Coupled Multispectroscopy Singular Laboratory (Raman-LASPEA) involves meeting the requirements set forth in the Protocol for access to SGIker and the services it provides.

Description

The combination of basic techniques and molecular spectroscopy is a powerful tool that has been successfully applied in conducting qualitative and quantitative analysis in fields as diverse as Earth Sciences, Environment, Fine Arts and / or Materials Sciences . The posibility to analyze both organic and inorganic compounds (solids, solutions, pastes, gels, films, etc.) as well as little or no sample preparation (nondestructive techniques or microinvasive) are some of the main advantages of this analytical methodology . The Singular Coupled Multispectroscopy Laboratory (Raman-LASPEA) is part of the General Research Services (SGIker) of the UPV / EHU and currently has the following instrumental techniques: FTIR imaging microscopy, confocal Raman microscopy, structural and chemical analyzer or SCA (combination electron microscopy, microanalysis or EDS , Raman spectroscopy and photoluminescence ), Raman imaging microscopy and atomic force microscopy (AFM-Raman and TERS).

Services

The infrastructure available at the Coupled Multispectroscopy Laboratory (Raman-LASPEA) enables analysis to be carried out in different technical-scientific areas. Any material with covalent links can be analysed using Raman and FRIT techniques. Some of the areas of application are listed below: Earth Sciences (Geochemistry) Mineral phase and rock characterisation Identifying precious stones Sludge study Fine Arts and Humanities Paints Agglutinants Sculptures Strategic cuts Sciences of the Materials Studying new polymers Inorganic compounds with special properties Nanoparticles Engineering and the Environment Pollution mechanisms and sources Monitoring chemical reactions and processes Catalyser characterisation and development Pre-history and Ancient History Studying supports Studying techniques to prepare archaeological materials Fossils Biomedicine Studying cancer cells

Location

University of the basque country UPV/EHU Medicine and odontology faculty Local 1R7 Barrio Sarriena s/n 48940 - Leioa (Bizkaia)

Equipment

- Three excitation lasers: 325, 514 and 785 nm - Ability to perform both puntual measurements as surface analysis by Raman image - Two external optical fiber probes allow analysis of macrosamples and a Linkam TS1500 temperature platform, obtain spectra of compounds at high temperatures This is an InVia Raman confocal microscope made by Renishaw, comprising a spectrometer and two lasers coupled to a Leica optical microscope. The excitation wavelengths of the lasers are 514 nm (green) and 785 nm (NIR), which enables analyses to be carried out using two different sources of energy, thus increasing the options for successful analysis and enabling possible fluorescence-based phenomena to be avoided. The spectrometer is equipped with two different modular optics depending on the laser being used. In all cases, a spectral resolution of 1 cm -1is obtained with a good signal-noise ratio. For its part, the Leica microscope incorporates 5x, 20x and 50x lenses, enabling maximum performance to be obtained from the laser photons in order to increase the sensitivity of the equipment by increasing the Raman effect. The confocality also enables maximum lateral resolution of the microscope to be obtained. The microscope incorporates a motorized XY slide for greater comfort and so as to be able to carry out Imagen Raman analyses. The Leica microscope may also be used as an optical micrscope, with reflected light and transmitted, etc., thus enabling any observations to be saved in digital format thanks to the video camera incorporated. The whole unit is placed on an optical desk to prevent any possible vibrations.
Renishaw InVia Raman
The laboratory is equipped with an FTIR spectrometer made by Jasco (4200 model) for determining FTIR spectres in medium infrared (400-4000 cm-1 ) using the potassium bromide pellet technique. The spectral resolution attained by the equipment is 0.5 cm-1.
Jasco 4200
The FTIR spectrometer is coupled to an FTIR scanning microscope made by Jasco (IMV 4000 model) and with motorized slide for carrying out FTIR Image analysis. The spectroscopic range scanned is from 4000 tp 750 cm-1 (limit determined by ZnSe optics). Its 16-detector online matrix enables analyses to be carried out in very short periods of time. Analyses may be carried out both in reflectance mode (16x y 32x), and transmittance mode (16x y 32x), as well as in ATR (16x).
IMV 4000
The laboratory has Carl-Zeiss EVO-40 Electronic Scanning Microscopy equipment at its disposal, whereby both photography in high vacuum mode (with metallization of the samples) and in extended pressure or low vacuum and wet sample mode (without the need to metallize the samples) can be used. The ewuipment features a secondary electron detector (SIM) and a retroscattered electron detector, together with an EDS elementary analyzer.
Carl-Zeiss EVO-40
The labatory has EDS microanalysis equipment made by Oxford Instruments at its disposal, coupled to the camera of the SEM microscope, thus enabling elementary analyses of the samples observed in the microscope to be carried out. The EDS equipment enables both specific analyses such as scanning and mapping to be carried out, thus also permitting the analysis of EDS image from both a qualitative and quantitative standpoint.
Oxford Instruments unknown model

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