Cell Analytics Facility

• Life & Medical Sciences

Laura Escobar (Facility Technician) laura.escobar@achucarro.org

For further details, please contact the center.

Cell Sorting

The Cell Sorting Facility is equiped with a BD FACSJazz (2B/4YG) cell sorter and analyser. This cell sorter incorporates some new design features (intuitive alignment, real-time video monitoring, drop-delay confirmation,...) that simplify the operation of stream-in-air machines, which helps increasing the operational efficiency for high workloads.

Main technical specifications

• Optics
  
  • 488 nm (blue) laser and 561 nm (yellow-green) laser independently aligned.
  • 20X, 0.6NA microscope objective.
  • Forward Scatter Detector and Filters: 75- and 50-mm lenses and PMT tube with a 488/10 BP filter and OD3 neutral density filter.
  • Side Scatter Detector and Filter: 90º collection lens and 488/10BP filter PMT.
  • Fluorescence Detectors and Filters: 90º collection lens and PMTs (2B/4YG).
• Fluidics
  
  • Sheath pressure fixed at 27 psi (1.8 bar) with a consumption of 0.5 L/h.
  • 100 µm nozzle with replaceable fluidics path and bubble detector.
• Performance
  
  • Two way sorting: 5 and 15 ml tubes.
  • Plates and slides: 6, 24, 96 and 384 well plates and user-defined collection devices.
  • Temperature control by circulating water bath.
  • Drop-delay confirmation (BD FACS Accudrop technology).
• Signal processing
  
  • Eight data acquisition channels.
  • Parallel data streams with channel ID and integrity check.
  • Less than 1 correlation error per 10⁸ events.
  • Maximum throughput rate: 200.000 events/second

Extracellular Flux Analysis

The Seahorse XF^e96 - Extracellular Flux Analyzer that can provide the value of the two major energy producing pathways of the cells: mitochondrial respiration and glycolysis, in a microplate and in real-time.

Microplate reader

The CLARIOstar Plus microplate reader is a flexible multi-mode reader. It comes equipped with the LVF Monochromators™, filters, and spectrometer. This flexibility is also combined with its sensitivity.

Science Park of the UPV/EHU, Sede Building, 3rd floorBarrio Sarriena, s/n, E-48940 Leioa (Spain)