- Life & Medical Sciences
Contac person: Javier Calvo
A high-resolution UHPLC–QTOF system with integrated ion mobility, designed to enhance the Mass Spectrometry Platform for advanced metabolomics, proteomics, and analytical chemistry applications.
Its key differentiating feature is the addition of a separation dimension (ion mobility), which enables the discrimination of isobaric ions and reduces interference in complex matrices while maintaining high resolution and accurate mass measurements.
The combination of UHPLC (with sub-2 µm columns), rapid acquisition, and high mass accuracy enables more robust molecular profiling, more reliable identification, and comparative quantification in demanding studies.
Equipment
- High-resolution UHPLC–QTOF system with integrated ion mobility.
- Quadrupole time-of-flight (QTOF) analyzer for high-resolution mass spectrometry.
- Dual ESI source with LockSpray/internal reference standard for accurate mass measurements.
- High-pressure UHPLC system (over 12,000 psi) for columns smaller than 2 µm.
- Rapid data acquisition (over 20 scans per second in conventional methods).
- Mass accuracy of less than 2 ppm with an internal reference standard.
- Dynamic range of at least 4 orders of magnitude.
- Qualitative and quantitative analysis of complex mixtures, including metabolomics, proteomics, and small molecules.
- Separation of isobaric ions using ion mobility to improve identification and reduce interference.
- High-resolution and accurate-mass measurements for molecular formula confirmation and compound annotation.
- LC-MS/MS analysis (depending on the method) for structural elucidation and verification.
- Screening and comparison of molecular profiles (targeted or untargeted, depending on the protocol).
- UHPLC method optimization using sub-2 µm columns and sample preparation.
- Support with experimental design, data interpretation, and reporting of results.
CIC biomaGUNE
Science and Technology Park of Gipuzkoa, Miramón 194, 20014 Donostia/San Sebastián, Gipuzkoa. Spain.
Tel.: +34 943 005 300
Biological and Preclinical Testing
Application sectors
- Pharmaceuticals and genetics
Industry plan
- HAZI · Biosanitary Sector
