- Life & Medical Sciences
Technical description of the instrumentation • Multiphoton scanning microscope: Leica SP5, Spectra-Physics Mai-Tai (pulsed Ti:sapphire laser tunable from 690 to 1040nm) • Incubator and chamber for living cell imaging during extended periods • High quantum efficiency GaAsP hybrid detectors and APDs for sensitive detection • TCSPC card: Becker & Hickl SPC 830 (FLIM, FCS and FCCS) • Analysis software: Imaris and Huygens. In-house developed analysis software • Storage and management of large volume of images: OMERO
Applications of interest • Spatio-temporal study of molecular interactions on the single cell basis (FRET-FLIM) • Intact-cell imaging of lipid concentration at the plasma membrane • Ligand-receptor binding (FCS) • Lipid-mediated protein interactions (scanning FCS) • Lipid packing in a membrane (Generalised Polarisation)
Instituto Biofisika, Campus Universitario de Leioa (University of the Basque Country UPV/EHU), Barrio Sarriena s/n, 48940 Leioa, Vizcaya (Spain).